PSEN1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA030760

 

Antibody CAB006844

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Chemicon
 

Product name

HPA030760 MAB5232 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known 

Released in version

7 3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

79  

Antigen sequence

EAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRS
TPESRAAVQELSSSILAGEDPEERGVKLG
 
 

Matching transcripts

PSEN1-001 - ENSP00000326366 [100%]
PSEN1-002 - ENSP00000350342 [100%]
PSEN1-003 - ENSP00000377719 [100%]
PSEN1-005 - ENSP00000385948 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity with a granular pattern in neuronal cells.
More information
 Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:35 1:500 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

52.7, 52.2, 42.1 52.7, 52.2, 46.3, 42.8, 42.3, 42.1, 21.1, 17.6, 16.6, 15.3, 12.5, 11.3, 10.6, 10.5, 7.3, 7.1, 6.7, 6.6, 4.9 

Antibody dilution

1:250 1:500 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:500  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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PSEN1-001
 
PSEN1-002
 
PSEN1-003
 
PSEN1-005