PPIE

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA020131

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA020131 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

91 

Antigen sequence

EEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAI
DNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKF
 

Matching transcripts

PPIE-001 - ENSP00000312769 [100%]
PPIE-002 - ENSP00000348904 [100%]
PPIE-003 - ENSP00000361918 [100%]
PPIE-009 - ENSP00000431714 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebellum shows strong nuclear positivity.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:75 

Literature conformity

Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNAseq data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404274)
More information
 

Target mass (kDa)

35.0, 33.4, 33.1, 22.8 

Antibody dilution

1:125 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PPIE-001
 
PPIE-002
 
PPIE-003
 
PPIE-009