GCDH

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA043252

 

Antibody HPA048492

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA043252 HPA048492 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

75 86 

Antigen sequence

SRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFH
REIISEMGELGVLGPTIKGYGCAGV
 
QYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAA
PEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYH
 

Matching transcripts

GCDH-001 - ENSP00000222214 [100%]
GCDH-002 - ENSP00000466845 [100%]
GCDH-010 - ENSP00000465770 [100%]
GCDH-202 - ENSP00000394872 [100%]
GCDH-015 - ENSP00000468584 [92%]
 GCDH-001 - ENSP00000222214 [100%]
GCDH-002 - ENSP00000466845 [100%]
GCDH-201 - ENSP00000394821 [100%]
GCDH-202 - ENSP00000394872 [100%]
GCDH-012 - ENSP00000468625 [94%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in granular pattern in hepatocytes.
More information
 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in granular pattern in hepatocytes.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:2500 1:35 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
More information
  

Antibody dilution

1:200  

Validation IF

Supportive: The subcellular location is supported by literature.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

48.1, 47.4, 18.4, 14.5 48.1, 47.4, 42.3, 12.7 

Antibody dilution

1:250 1:200 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:12000 1:1600 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
GCDH-001
 
GCDH-002
 
GCDH-010
 
GCDH-012
 
GCDH-015
 
GCDH-201
 
GCDH-202