PRPF31

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA041939

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA041939 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

8 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

103 

Antigen sequence

TNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVE
SRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTL
SGF
 

Matching transcripts

PRPF31-001 - ENSP00000324122 [100%]
PRPF31-003 - ENSP00000375635 [100%]
PRPF31-005 - ENSP00000395894 [100%]
PRPF31-006 - ENSP00000408980 [100%]
PRPF31-201 - ENSP00000405166 [100%]
PRPF31-009 - ENSP00000395089 [99%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and islets of Langerhans.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:140 

Validation IH

Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

55.5, 54.8, 50.2, 31.4, 30.6, 28.9 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:6000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PRPF31-001
 
PRPF31-003
 
PRPF31-005
 
PRPF31-006
 
PRPF31-009
 
PRPF31-201