FKBP8

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA045177

 

Antibody CAB025346

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 R&D Systems
 

Product name

HPA045177 MAB3580 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

12 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein 

Length (aa)

138  

Antigen sequence

YDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALR
SCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIH
AELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGK
 
 

Matching transcripts

FKBP8-001 - ENSP00000471700 [100%]
FKBP8-002 - ENSP00000472302 [100%]
FKBP8-014 - ENSP00000476767 [100%]
FKBP8-016 - ENSP00000222308 [100%]
FKBP8-201 - ENSP00000388891 [100%]
FKBP8-004 - ENSP00000441267 [91%]
FKBP8-202 - ENSP00000476586 [91%]
FKBP8-011 - ENSP00000475588 [81%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells and in neuropil.
More information
 Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:100 1:3500 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Consistent with RNAseq data Mainly consistent with RNAseq data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & mitochondria.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
More information
 

Antibody dilution

1:36 1:2000 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

47.1, 44.6, 27.5, 27.1, 19.9 47.1, 44.6, 27.5, 27.1, 21.8, 19.9, 17.9, 17.4, 12.2, 11.0, 2.3 

Antibody dilution

1:250 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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FKBP8-001
 
FKBP8-002
 
FKBP8-004
 
FKBP8-011
 
FKBP8-014
 
FKBP8-016
 
FKBP8-201
 
FKBP8-202