CLDN15

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA053199

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA053199 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

22 

Antigen sequence

WYAFNITRDFFDPLYPGTKYEL
 

Matching transcripts

CLDN15-001 - ENSP00000385300 [100%]
CLDN15-002 - ENSP00000308870 [100%]
CLDN15-006 - ENSP00000398418 [100%]
CLDN15-007 - ENSP00000390230 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells, endothelial cells and ganglion were moderately positive.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:175 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

24.4, 18.1, 16.1 

Antibody dilution

1:580 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:4600 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CLDN15-001
 
CLDN15-002
 
CLDN15-006
 
CLDN15-007