TGFBR1

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA056473

 

Antibody CAB002441

 

Antibody CAB031481

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Novocastra
 Santa Cruz Biotechnology
 

Product name

HPA056473 NCL-TGFBR1 sc-402 

Host species

Rabbit Mouse Rabbit 

Clonality

pAb mAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known Not known 

Released in version

13 6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known Synthetic peptide 

Length (aa)

32   

Antigen sequence

ICHNRTVIHHRVPNEEDPSLDRPFISEGTTLK
 
 
 

Matching transcripts

TGFBR1-001 - ENSP00000364133 [100%]
TGFBR1-004 - ENSP00000447297 [100%]
TGFBR1-005 - ENSP00000446685 [100%]
TGFBR1-007 - ENSP00000450052 [100%]
TGFBR1-008 - ENSP00000449934 [100%]
TGFBR1-009 - ENSP00000447182 [100%]
TGFBR1-011 - ENSP00000448518 [100%]
TGFBR1-012 - ENSP00000447707 [100%]
   

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Application not done for this antibody. Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
More information
 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

 HIER pH6 HIER pH6 

Antibody dilution

 1:50 1:300 

Literature conformity

 Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

 Not done Not done 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

56.3, 56.0, 48.9, 41.8, 20.6, 13.6, 13.0, 12.4 56.3, 56.0, 48.9, 47.7, 41.8, 20.6, 13.6, 13.1, 13.0, 12.4 56.3, 56.0, 48.9, 47.7, 41.8, 20.6, 13.6, 13.1, 13.0, 12.4 

Antibody dilution

1:280 1:500 1:500 

Validation WB

Uncertain: No bands detected Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line A549 shows positivity in vesicles.
More information
 Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:85   

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.   

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:3400   

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.   
 

ANTIGEN VIEW

? »
 
 
 
TGFBR1-001
 
TGFBR1-004
 
TGFBR1-005
 
TGFBR1-007
 
TGFBR1-008
 
TGFBR1-009
 
TGFBR1-011
 
TGFBR1-012