RAPGEFL1

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA022895

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA022895 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

96 

Antigen sequence

MEGPEGLGRKQACLAMLLHFLDTYQGLLQEEEGAGHIIKDLYLLIMKDES
LYQGLREDTLRLHQLVETVELKIPEENQPPSKQVKPLFRHFRRIDS
 

Matching transcripts

RAPGEFL1-004 - ENSP00000438631 [100%]
RAPGEFL1-005 - ENSP00000394530 [100%]
RAPGEFL1-007 - ENSP00000264644 [100%]
RAPGEFL1-008 - ENSP00000441059 [100%]
RAPGEFL1-011 - ENSP00000440226 [100%]
RAPGEFL1-201 - ENSP00000408322 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in subsets of tubular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:35 

Literature conformity

Not done 

RNA consistency

Not at all consistent with RNAseq data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in microtubules & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:20 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

58.8, 58.0, 52.3, 17.2, 11.8 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
RAPGEFL1-004
 
RAPGEFL1-005
 
RAPGEFL1-007
 
RAPGEFL1-008
 
RAPGEFL1-011
 
RAPGEFL1-201