RPS6KB1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA039442

 

Antibody CAB003838

 

Antibody CAB018346

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Epitomics
 Santa Cruz Biotechnology
 

Product name

HPA039442 1494-1 sc-230 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb mAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Supernatant Affinity 

Released in version

13 2 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide Synthetic peptide 

Length (aa)

71   

Antigen sequence

VSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAP
LPIRQPNSGPYKKQAFPMISK
 
 
 

Matching transcripts

RPS6KB1-001 - ENSP00000225577 [100%]
RPS6KB1-008 - ENSP00000441993 [100%]
RPS6KB1-201 - ENSP00000376744 [100%]
   

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.
More information
 Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
More information
 Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:100 1:150 1:65 

Literature conformity

Consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not done Mainly consistent with RNAseq data Mainly consistent with RNAseq data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

59.1, 56.6, 53.0 59.1, 56.6, 53.0, 51.0, 15.8, 6.9, 6.0 59.1, 56.6, 53.0, 51.0, 15.8, 6.9, 6.0 

Antibody dilution

1:250 1:500 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm, the Golgi apparatus & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:29  1:16 

Validation IF

Supportive: The subcellular location is supported by literature.  Supportive: The subcellular location is supported by literature. 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:3000   

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).   
 

ANTIGEN VIEW

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RPS6KB1-001
 
RPS6KB1-008
 
RPS6KB1-201