ALDH3A1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051150

 

Antibody HPA063783

 

Antibody CAB045957

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Origene
 

Product name

HPA051150 HPA063783 TA501139 

Host species

Rabbit Rabbit Mouse 

Clonality

pAb pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

11 13 11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein 

Length (aa)

47 54  

Antigen sequence

NSGMGSYHGKKSFETFSHRRSCLVRPLMNDEGLKVRYPPSPAKMTQH
 
LHKNEWNAYYEEVVYVLEEIEYMIQKLPEWAADEPVEKTPQTQQDELYIH
SEPL
 
 

Matching transcripts

ALDH3A1-001 - ENSP00000225740 [100%]
ALDH3A1-005 - ENSP00000460482 [100%]
ALDH3A1-007 - ENSP00000378923 [100%]
ALDH3A1-010 - ENSP00000411821 [100%]
ALDH3A1-201 - ENSP00000388469 [100%]
 ALDH3A1-001 - ENSP00000225740 [100%]
ALDH3A1-006 - ENSP00000434685 [100%]
ALDH3A1-007 - ENSP00000378923 [100%]
ALDH3A1-009 - ENSP00000389766 [100%]
ALDH3A1-010 - ENSP00000411821 [100%]
ALDH3A1-013 - ENSP00000458841 [100%]
ALDH3A1-016 - ENSP00000461189 [100%]
ALDH3A1-201 - ENSP00000388469 [100%]
ALDH3A1-018 - ENSP00000388369 [94%]
  

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and nuclear positivity in glandular cells.
More information
 Application not done for this antibody. Immunohistochemical staining of human esophagus shows strong cytoplasmic and nuclear staining in squamous epithelial cells.
More information
 

Retrieval method

HIER pH6  HIER pH6 

Antibody dilution

1:600  1:30000 

Literature conformity

Consistent with extensive gene/protein characterization data  Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNAseq data  Mainly consistent with RNAseq data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

50.4, 43.3, 41.9 50.4, 44.2, 43.3, 41.6, 29.1, 15.0, 14.0 50.4, 44.2, 43.3, 41.9, 41.6, 29.1, 15.0, 14.0, 8.7 

Antibody dilution

1:250 1:240 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line A549 shows positivity in plasma membrane & cytoplasm.
More information
 Immunofluorescent staining of human cell line A549 shows positivity in cytoplasm, vesicles & nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

1:50 1:73  

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature.  

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:2000 1:2950  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

? »
 
 
 
ALDH3A1-001
 
ALDH3A1-005
 
ALDH3A1-006
 
ALDH3A1-007
 
ALDH3A1-009
 
ALDH3A1-010
 
ALDH3A1-013
 
ALDH3A1-016
 
ALDH3A1-018
 
ALDH3A1-201