CD5

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB015392

 

Antibody CAB020308

 

ANTIBODY INFORMATION

Provider

Santa Cruz Biotechnology
 Lab Vision/NeoMarkers
 

Product name

sc-53204 RM-9119 

Host species

Mouse Rabbit 

Clonality

mAb mAb 

Purity

Protein A/G Not known 

Released in version

4 5 

ANTIGEN INFORMATION

Antigen

Synthetic peptide Synthetic peptide 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.
More information
 Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells outside the reaction centra.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:50 1:100 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows no positivity.
More information
 Immunofluorescent staining of human cell line U-251MG shows no positivity.
More information
 

Antibody dilution

1:50 1:100 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

54.6, 18.4 54.6, 18.4 

Antibody dilution

1:500 1:500 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 
 

ANTIGEN VIEW

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 Not available