METAP2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA019095

 

Antibody CAB013025

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Zymed
 

Product name

HPA019095 71-7200 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known 

Released in version

5 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known 

Length (aa)

92  

Antigen sequence

SGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKE
SGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKK
 
 

Matching transcripts

METAP2-001 - ENSP00000325312 [100%]
METAP2-003 - ENSP00000446052 [100%]
METAP2-004 - ENSP00000448169 [100%]
METAP2-007 - ENSP00000450063 [99%]
METAP2-002 - ENSP00000261220 [83%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
More information
 Immunohistochemical staining of human gall bladder shows cytoplasmic positivity in glandular cells and macrophages.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:2000 1:200 

Validation IH

Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

52.9, 52.8, 50.5, 36.0 52.9, 52.8, 50.5, 48.9, 36.0, 19.7, 18.1, 17.1 

Antibody dilution

1:250 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Only bands not corresponding to the predicted size 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
More information
  

Antibody dilution

1:200  

Validation IF

Supportive: The subcellular location is supported by literature.  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:12000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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METAP2-001
 
METAP2-002
 
METAP2-003
 
METAP2-004
 
METAP2-007