WWC1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038016

 

Antibody HPA038017

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038016 HPA038017 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

80 67 

Antigen sequence

RSGERSTRWYNLLSYKYLKKQSRELKPVGVMAPASGPASTDAVSALLEQT
AVELEKRQEGRSSTQTLEDSWRYEETSENE
 
PPCSPLMADPLLAGDAFLNSLEFEDPELSATLCELSLGNSAQERYRLEEP
GTEGKQLGQAVNTAQGC
 

Matching transcripts

WWC1-001 - ENSP00000265293 [100%]
WWC1-002 - ENSP00000427772 [100%]
WWC1-004 - ENSP00000377473 [100%]
WWC1-006 - ENSP00000429339 [100%]
WWC1-007 - ENSP00000428084 [100%]
 WWC1-001 - ENSP00000265293 [100%]
WWC1-002 - ENSP00000427772 [100%]
WWC1-004 - ENSP00000377473 [100%]
WWC1-006 - ENSP00000429339 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
More information
 Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:700 1:100 

Literature conformity

Partly consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in the Golgi apparatus.
More information
 Application not done for this antibody. 

Antibody dilution

1:145  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

125.9, 125.3, 121.1, 99.0, 50.6 125.9, 125.3, 121.1, 99.0 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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WWC1-001
 
WWC1-002
 
WWC1-004
 
WWC1-006
 
WWC1-007