GNB4

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA040736

 

Antibody CAB018383

 

Antibody CAB018737

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 Santa Cruz Biotechnology
 

Product name

HPA040736 sc-378 sc-25413 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb msAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity Protein A/G 

Released in version

10 5 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide Recombinant protein 

Length (aa)

29   

Antigen sequence

CRLFDLRADQELLLYSHDNIICGITSVAF
 
 
 

Matching transcripts

GNB4-001 - ENSP00000232564 [100%]
GNB4-003 - ENSP00000419693 [100%]
   

Other gene match

GNB1 - ENSG00000078369 [90%]
GNB3 - ENSG00000111664 [83%]
GNB2 - ENSG00000172354 [97%]
   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human colon shows moderate membranous positivity in glandular cells.
More information
 Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:200 1:7500 1:50 

Validation IH

Supportive: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for all of the genes Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

37.6, 37.4, 37.3, 37.2, 37.1, 32.4, 29.3, 25.9 37.6, 17.9, 9.1 37.6, 17.9, 9.1 

Antibody dilution

1:250 1:500 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
   

Antibody dilution

1:500   

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.   
 

ANTIGEN VIEW

? »
 
 
 
GNB4-001
 
GNB4-003