RNF146

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA027158

 

Antibody HPA027209

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA027158 HPA027209 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

74 87 

Antigen sequence

IPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELED
AFSKGKKNTEMLIAGFLYVADLEN
 
MVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDANTVNLARESSADGA
DSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPD
 

Matching transcripts

RNF146-001 - ENSP00000357297 [100%]
RNF146-002 - ENSP00000349253 [100%]
RNF146-201 - ENSP00000309365 [100%]
 RNF146-001 - ENSP00000357297 [100%]
RNF146-002 - ENSP00000349253 [100%]
RNF146-201 - ENSP00000309365 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human thyroid gland shows distinct nuclear and cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:20 1:400 

Validation IH

Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
More information
 Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cytoplasm.
More information
 

Antibody dilution

1:10 1:200 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

38.9, 38.8 38.9, 38.8 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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RNF146-001
 
RNF146-002
 
RNF146-201