MYL12B

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA039262

 

Antibody HPA045244

 

Antibody HPA059197

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA039262 HPA045244 HPA059197 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 10 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

25 30 27 

Antigen sequence

MFEQTQIQEFKEAFTIMDQNRDGFI
 
YREAPIDKKGNFNYIEFTRILKHGAKDKDD
 
MSSKRTKTKTKKRPQRATSNVFAMFDQ
 

Matching transcripts

MYL12B-001 - ENSP00000237500 [80%]
MYL12B-002 - ENSP00000463559 [80%]
MYL12B-003 - ENSP00000383037 [80%]
MYL12B-004 - ENSP00000464464 [80%]
 MYL12B-001 - ENSP00000237500 [100%]
MYL12B-002 - ENSP00000463559 [100%]
MYL12B-003 - ENSP00000383037 [100%]
MYL12B-004 - ENSP00000464464 [100%]
 MYL12B-001 - ENSP00000237500 [89%]
MYL12B-002 - ENSP00000463559 [89%]
MYL12B-003 - ENSP00000383037 [89%]
MYL12B-004 - ENSP00000464464 [89%]
 

Other gene match

MYL9 - ENSG00000101335 [80%]
MYL12A - ENSG00000101608 [80%]
MYL7 - ENSG00000106631 [80%]
MYL2 - ENSG00000111245 [100%]
MYLPF - ENSG00000180209 [84%]
MYL5 - ENSG00000215375 [92%]
 MYL9 - ENSG00000101335 [97%]
MYL12A - ENSG00000101608 [100%]
 MYL9 - ENSG00000101335 [89%]
MYL12A - ENSG00000101608 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
More information
 Immunohistochemical staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
More information
 Immunohistochemical staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:150 1:450 1:75 

Literature conformity

Consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNAseq data Consistent with RNAseq data Mainly consistent with RNAseq data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400153)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

22.0, 20.5, 19.8, 19.5, 19.4, 19.0, 18.8, 18.5, 16.7, 15.2, 14.1, 13.9, 13.2, 13.1, 12.0 20.5, 19.8, 13.9 20.5, 19.8, 13.9, 13.1 

Antibody dilution

1:250 1:250 1:110 

Validation WB

Uncertain: No bands detected Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 1:500 1:1150 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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MYL12B-001
 
MYL12B-002
 
MYL12B-003
 
MYL12B-004