MYL12B

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA039262

 

Antibody HPA045244

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA039262 HPA045244 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

25 30 

Antigen sequence

MFEQTQIQEFKEAFTIMDQNRDGFI
 
YREAPIDKKGNFNYIEFTRILKHGAKDKDD
 

Matching transcripts

MYL12B-001 - ENSP00000237500 [80%]
MYL12B-002 - ENSP00000463559 [80%]
MYL12B-003 - ENSP00000383037 [80%]
MYL12B-004 - ENSP00000464464 [80%]
 MYL12B-001 - ENSP00000237500 [100%]
MYL12B-002 - ENSP00000463559 [100%]
MYL12B-003 - ENSP00000383037 [100%]
MYL12B-004 - ENSP00000464464 [100%]
 

Other gene match

MYL9 - ENSG00000101335 [80%]
MYL12A - ENSG00000101608 [80%]
MYL7 - ENSG00000106631 [80%]
MYL2 - ENSG00000111245 [100%]
MYLPF - ENSG00000180209 [84%]
MYL5 - ENSG00000215375 [92%]
 MYL9 - ENSG00000101335 [97%]
MYL12A - ENSG00000101608 [100%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
More information
 Immunohistochemical staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:450 

Validation IH

Supportive: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for all of the genes Uncertain: The multi-targeting antibody yielding a staining pattern partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data for all of the genes 

         Western Blot

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Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400153)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

22.0, 20.5, 19.8, 19.5, 19.4, 19.0, 18.8, 18.5, 16.7, 15.2, 14.1, 13.9, 13.2, 13.1, 12.0 20.5, 19.8, 13.9 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 1:500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MYL12B-001
 
MYL12B-002
 
MYL12B-003
 
MYL12B-004