The detailed normal tissue page shows images of the stained tissue, together with antibody staining/expression level of the cell types.
If a knowledge-based annotated protein expression is provided, this is reported for each cell
type at the top of the page and the staining level of the individual antibodies is given underneath each antibody ID.
Samples from up to three different individuals have been stained for each antibody. The gender, age and tissue characterization
are reported for each individual (patient) and are viewable when clicking on the image for magnification.
The images can be clicked for an enlarged view that can be panned. From the enlarged view, all stained images for all antibodies can be browsed (represented by miniature images). The miniature image with a green overlay is the currently displayed image.
The RNAseq details section shows detailed information about the individual samples used for the transcript profiling and results of the RNAseq analysis.
On top the transcript layout for all protein-coding transcripts of the gene, with introns scaled down by a factor 20, is shown. Information about each individual sample is listed below, including gender, age, a tissue section image and estimated fractions of cell types. FPKM (Fragments Per Kilobase of exon per Million fragments mapped) values give a quantification of the gene abundance which is comparable between different genes and samples.
The plots show the mapped read coverage along all exons and introns of the gene, thereby revealing which exons are expressed and at what relative levels. The levels are normalized by total library size and scaled to fit the highest peak of all samples to the height of the plot. Thus these plots should not be used to compare abundances between different samples or genes, but rather as an indication of which transcripts are expressed and as a quality control.
Female, age 60
Sample 1 FPKM: 3.8
Male, age 54
Sample 2 FPKM: 2.9
Hepatocytes: 80 Fibroblasts: 15 Other cell types: 5
Male, age 74
Sample 3 FPKM: 7.2
Hepatocytes: 85 Fibroblasts: 5 Other cell types: 10
The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.