CCRL2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA043238

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA043238 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

8 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

43 

Antigen sequence

FLDGTFSKYLCRCFHLRSNTPLQPRGQSAQGTSREEPDHSTEV
 

Matching transcripts

CCRL2-001 - ENSP00000349967 [100%]
CCRL2-002 - ENSP00000381994 [100%]
CCRL2-003 - ENSP00000383678 [100%]
CCRL2-004 - ENSP00000383677 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp and cells in white pulp.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:700 

Literature conformity

Partly consistent with gene/protein characterization data 

RNA consistency

Mainly consistent with RNAseq data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

41.0, 39.5 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:12000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CCRL2-001
 
CCRL2-002
 
CCRL2-003
 
CCRL2-004