HACL1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035496

 

Antibody HPA035497

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035496 HPA035497 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

89 95 

Antigen sequence

CVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKF
QDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQ
 
YGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASV
IRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVV
 

Matching transcripts

HACL1-001 - ENSP00000323811 [100%]
HACL1-002 - ENSP00000403656 [100%]
HACL1-003 - ENSP00000395278 [100%]
HACL1-005 - ENSP00000390699 [100%]
HACL1-007 - ENSP00000404883 [100%]
 HACL1-001 - ENSP00000323811 [100%]
HACL1-005 - ENSP00000390699 [100%]
HACL1-007 - ENSP00000404883 [100%]
HACL1-008 - ENSP00000391393 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:25 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Mainly consistent with RNAseq data Mainly consistent with RNAseq data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

63.7, 61.1, 56.7, 54.7, 37.6 63.7, 61.1, 56.7, 26.4 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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HACL1-001
 
HACL1-002
 
HACL1-003
 
HACL1-005
 
HACL1-007
 
HACL1-008