MCCC2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038300

 

Antibody HPA038301

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038300 HPA038301 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

86 77 

Antigen sequence

GIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWP
NARISVMGGEQAANVLATITKDQRAREGKQFSSADE
 
LHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGI
GRVSGVECMIIANDATVKGGAYYPVTV
 

Matching transcripts

MCCC2-001 - ENSP00000343657 [100%]
MCCC2-009 - ENSP00000425474 [100%]
MCCC2-201 - ENSP00000327308 [100%]
 MCCC2-001 - ENSP00000343657 [100%]
MCCC2-002 - ENSP00000423202 [100%]
MCCC2-003 - ENSP00000420994 [100%]
MCCC2-201 - ENSP00000327308 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
More information
  

Antibody dilution

1:105  

Validation IF

Supportive: The subcellular location is supported by literature.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

61.3, 57.5, 31.6 61.3, 57.5, 39.7, 36.3 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:250 

Validation IH

 Supportive: Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 
 

ANTIGEN VIEW

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MCCC2-001
 
MCCC2-002
 
MCCC2-003
 
MCCC2-009
 
MCCC2-201