PSRC1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA049315

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA049315 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

77 

Antigen sequence

DLEEDVRFIVDETLDFGGLSPSDSREEEDITVLVTPEKPLRRGLSHRSDP
NAVAPAPQGVRLSLGPLSPEKLEEILD
 

Matching transcripts

PSRC1-001 - ENSP00000358923 [100%]
PSRC1-002 - ENSP00000358920 [100%]
PSRC1-003 - ENSP00000474667 [100%]
PSRC1-005 - ENSP00000387791 [100%]
PSRC1-006 - ENSP00000413186 [100%]
PSRC1-009 - ENSP00000386323 [100%]
PSRC1-010 - ENSP00000358919 [100%]
PSRC1-201 - ENSP00000358925 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:400 

Literature conformity

Partly consistent with gene/protein characterization data 

RNA consistency

Mainly not consistent with RNA expression data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

38.8, 35.6, 33.6, 25.0, 22.3 

Antibody dilution

1:100 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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PSRC1-001
 
PSRC1-002
 
PSRC1-003
 
PSRC1-005
 
PSRC1-006
 
PSRC1-009
 
PSRC1-010
 
PSRC1-201