CMTM3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA013870

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA013870 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

30 

Antigen sequence

NDVAKFLKQGDSADETTAHKTEEENSDSDS
 

Matching transcripts

CMTM3-001 - ENSP00000400482 [100%]
CMTM3-002 - ENSP00000461758 [100%]
CMTM3-004 - ENSP00000354579 [100%]
CMTM3-006 - ENSP00000455851 [100%]
CMTM3-008 - ENSP00000455758 [100%]
CMTM3-009 - ENSP00000457923 [100%]
CMTM3-011 - ENSP00000457589 [100%]
CMTM3-018 - ENSP00000461654 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:300 

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
More information
 

Antibody dilution

1:43 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405702)
More information
 

Target mass (kDa)

19.7, 16.4, 10.6 

Antibody dilution

1:125 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CMTM3-001
 
CMTM3-002
 
CMTM3-004
 
CMTM3-006
 
CMTM3-008
 
CMTM3-009
 
CMTM3-011
 
CMTM3-018