MBD1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB009017

 

Antibody CAB036003

 

ANTIBODY INFORMATION

Provider

Santa Cruz Biotechnology
 NCI-CPTC
 

Product name

sc-10751 CPTC-MBD1-1 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Protein A/G Protein A/G 

Released in version

4 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein Recombinant protein 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human small intestine shows distinct nuclear and cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human kidney shows strong cytoplasmic, nuclear and luminal membranous positivity in tubular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:200 1:10000 

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus.
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Antibody dilution

1:50  

Validation IF

Supportive: The subcellular location is supported by literature.  

         Western Blot

Image

  

Description

  

Target mass (kDa)

72.4, 72.1, 69.6, 66.6, 64.7, 62.6, 62.4, 61.3, 60.8, 60.0, 59.2, 58.8, 55.2, 45.8, 28.2, 25.7, 5.1 72.4, 72.1, 69.6, 66.6, 64.7, 62.6, 62.4, 61.3, 60.8, 60.0, 59.2, 58.8, 55.2, 45.8, 28.2, 25.7, 5.1 

Antibody dilution

1:500 1:500 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 
 

ANTIGEN VIEW

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 Not available