ENSA

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA051294

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA051294 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

36 

Antigen sequence

MSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAK
 

Matching transcripts

ENSA-001 - ENSP00000358010 [100%]
ENSA-002 - ENSP00000271690 [100%]
ENSA-003 - ENSP00000355220 [100%]
ENSA-004 - ENSP00000358012 [100%]
ENSA-006 - ENSP00000341743 [100%]
ENSA-010 - ENSP00000348921 [100%]
ENSA-012 - ENSP00000421458 [100%]
ENSA-013 - ENSP00000426110 [100%]
ENSA-015 - ENSP00000424242 [100%]
ENSA-201 - ENSP00000358005 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:300 

Literature conformity

No avaliable characterization data 

RNA consistency

Consistent with RNAseq data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line PC-3 shows positivity in nucleus & nucleoli.
More information
 

Antibody dilution

1:68 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

21.0, 16.0, 15.6, 15.3, 14.9, 13.4, 13.0, 12.0 

Antibody dilution

1:340 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2700 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
ENSA-001
 
ENSA-002
 
ENSA-003
 
ENSA-004
 
ENSA-006
 
ENSA-010
 
ENSA-012
 
ENSA-013
 
ENSA-015
 
ENSA-201