RNF145

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA036562

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA036562 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

84 

Antigen sequence

NSSQLPGLGTEPVLQPHAGAEQNVMFQEGTEPPGQEHTPGTRIQEGSRDN
NEYIARRPDNQEGAFDPKEYPHSAKDEAHPVESA
 

Matching transcripts

RNF145-001 - ENSP00000274542 [100%]
RNF145-002 - ENSP00000409064 [100%]
RNF145-003 - ENSP00000430955 [100%]
RNF145-004 - ENSP00000429071 [100%]
RNF145-005 - ENSP00000430397 [100%]
RNF145-006 - ENSP00000430753 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:700 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
More information
 

Antibody dilution

1:98 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

 

Target mass (kDa)

79.2, 78.9, 77.6, 77.3, 75.6 

Antibody dilution

1:250 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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RNF145-001
 
RNF145-002
 
RNF145-003
 
RNF145-004
 
RNF145-005
 
RNF145-006