CREB5

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA050020

 

Antibody HPA061622

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA050020 HPA061622 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

14 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

68 31 

Antigen sequence

PAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPHHPYPHQ
HQHPAHHPHPQPHHQQNH
 
SSPPASPVPACSQQQVIQHNTITTSSSVSEV
 

Matching transcripts

CREB5-001 - ENSP00000350359 [100%]
CREB5-002 - ENSP00000379594 [100%]
CREB5-003 - ENSP00000379592 [100%]
CREB5-004 - ENSP00000387197 [100%]
CREB5-005 - ENSP00000379593 [100%]
CREB5-012 - ENSP00000394979 [100%]
 CREB5-001 - ENSP00000350359 [100%]
CREB5-002 - ENSP00000379594 [100%]
CREB5-003 - ENSP00000379592 [100%]
CREB5-004 - ENSP00000387197 [100%]
CREB5-005 - ENSP00000379593 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line HEK 293 shows positivity in nucleus & plasma membrane.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:54 1:10 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

56.9, 56.0, 53.1, 41.2, 24.0 56.9, 56.0, 53.1, 41.2 

Antibody dilution

1:270 1:80 

Validation WB

Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2150 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CREB5-001
 
CREB5-002
 
CREB5-003
 
CREB5-004
 
CREB5-005
 
CREB5-012