CREB5

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA050020

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA050020 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

68 

Antigen sequence

PAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPHHPYPHQ
HQHPAHHPHPQPHHQQNH
 

Matching transcripts

CREB5-001 - ENSP00000350359 [100%]
CREB5-002 - ENSP00000379594 [100%]
CREB5-003 - ENSP00000379592 [100%]
CREB5-004 - ENSP00000387197 [100%]
CREB5-005 - ENSP00000379593 [100%]
CREB5-012 - ENSP00000394979 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line HEK 293 shows positivity in nucleus & plasma membrane.
More information
 

Antibody dilution

1:54 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

56.9, 56.0, 53.1, 41.2, 24.0 

Antibody dilution

1:270 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2150 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CREB5-001
 
CREB5-002
 
CREB5-003
 
CREB5-004
 
CREB5-005
 
CREB5-012