C9orf3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA004633

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA004633 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

133 

Antigen sequence

ECSSWYYYVTMPMPASTFTIAVGCWTEMKMETWSSNDLATERPFSPSEAN
FRHVGVCSHMEYPCRFQNASATTQEIIPHRVFAPVCLTGACQETLLRLIP
PCLSAAHSVLGAHPFSRLDVLIVPANFPSLGMA
 

Matching transcripts

C9orf3-002 - ENSP00000297979 [100%]
C9orf3-004 - ENSP00000277198 [100%]
C9orf3-006 - ENSP00000402171 [100%]
C9orf3-008 - ENSP00000401854 [100%]
C9orf3-201 - ENSP00000364464 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:25 

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409911)
More information
 

Target mass (kDa)

93.6, 82.1, 69.2, 63.4, 58.3 

Antibody dilution

1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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C9orf3-002
 
C9orf3-004
 
C9orf3-006
 
C9orf3-008
 
C9orf3-201