EI24

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA047165

 

Antibody HPA051029

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA047165 HPA051029 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

57 31 

Antigen sequence

GIKDSIWGICTISKLDARIQQKREEQRRRRASSVLAQRRAQSIERKQESE
PRIVSRI
 
HKTVYLQSALSSSTSAEKFPSPHPSPAKLKA
 

Matching transcripts

EI24-008 - ENSP00000433376 [100%]
EI24-009 - ENSP00000435034 [100%]
EI24-201 - ENSP00000278903 [100%]
EI24-202 - ENSP00000364081 [100%]
EI24-011 - ENSP00000435923 [84%]
 EI24-201 - ENSP00000278903 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:400  

Literature conformity

Consistent with gene/protein characterization data  

RNA consistency

Consistent with RNAseq data  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417681)
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417681)
More information
 

Target mass (kDa)

38.9, 30.0, 25.1, 21.6, 14.6 38.9 

Antibody dilution

1:250 1:125 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:6700 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & endoplasmic reticulum.
More information
 

Antibody dilution

 1:5 

Validation IF

 Supportive: The subcellular location is supported by literature. 
 

ANTIGEN VIEW

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EI24-008
 
EI24-009
 
EI24-011
 
EI24-201
 
EI24-202