CHEK1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA044364

 

Antibody CAB002049

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Novocastra
 

Product name

HPA044364 NCL-Chk1 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known 

Released in version

13 1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known 

Length (aa)

130  

Antigen sequence

KRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLN
PWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVT
SGGVSESPSGFSKHIQSNLDFSPVNSASSE
 
 

Matching transcripts

CHEK1-001 - ENSP00000391090 [100%]
CHEK1-002 - ENSP00000412504 [100%]
CHEK1-004 - ENSP00000435371 [100%]
CHEK1-006 - ENSP00000278916 [100%]
CHEK1-007 - ENSP00000432890 [100%]
CHEK1-201 - ENSP00000388648 [100%]
CHEK1-202 - ENSP00000442317 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Application not done for this antibody. Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:50 

Literature conformity

 Partly consistent with extensive gene/protein characterization data 

RNA consistency

 Not done 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

55.5, 54.4, 50.4, 49.0 55.5, 54.4, 50.4, 49.0, 23.3, 22.3, 19.5, 15.2, 14.4, 4.6 

Antibody dilution

1:250 1:500 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Uncertain: No bands detected 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

1:61  

Validation IF

Supportive: The subcellular location is supported by literature.  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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CHEK1-001
 
CHEK1-002
 
CHEK1-004
 
CHEK1-006
 
CHEK1-007
 
CHEK1-201
 
CHEK1-202