ALG8

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051898

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA051898 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

24 

Antigen sequence

AVRECCKCIDGKKVGKELTEKPKF
 

Matching transcripts

ALG8-001 - ENSP00000299626 [100%]
ALG8-004 - ENSP00000437199 [100%]
ALG8-005 - ENSP00000365326 [100%]
ALG8-009 - ENSP00000436064 [100%]
ALG8-010 - ENSP00000435467 [100%]
ALG8-011 - ENSP00000435417 [100%]
ALG8-012 - ENSP00000434660 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:125 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
More information
 

Antibody dilution

1:27 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

60.1, 53.2, 32.7, 28.3, 27.3, 19.8, 15.9 

Antibody dilution

1:130 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1050 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ALG8-001
 
ALG8-004
 
ALG8-005
 
ALG8-009
 
ALG8-010
 
ALG8-011
 
ALG8-012