BDH1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA030947

 

Antibody HPA058709

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA030947 HPA058709 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

89 73 

Antigen sequence

ATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGST
DTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHL
 
DSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLN
VCSSEEVEKVVEIVRSSLKDPEK
 

Matching transcripts

BDH1-001 - ENSP00000376183 [100%]
BDH1-002 - ENSP00000411014 [100%]
BDH1-201 - ENSP00000350914 [100%]
BDH1-202 - ENSP00000376184 [100%]
 BDH1-001 - ENSP00000376183 [100%]
BDH1-006 - ENSP00000409849 [100%]
BDH1-011 - ENSP00000408685 [100%]
BDH1-201 - ENSP00000350914 [100%]
BDH1-202 - ENSP00000376184 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity with a granular pattern in hepatocytes.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:1000  

Literature conformity

Consistent with gene/protein characterization data  

RNA consistency

Mainly consistent with RNAseq data  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in mitochondria & nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line MCF7 shows positivity in mitochondria.
More information
 

Antibody dilution

1:116 1:6 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

38.2, 29.0 38.2, 20.3, 18.4 

Antibody dilution

1:250 1:80 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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BDH1-001
 
BDH1-002
 
BDH1-006
 
BDH1-011
 
BDH1-201
 
BDH1-202