ANKRD33B

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA056974

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA056974 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

71 

Antigen sequence

TALMKAAMQGRTDCIRALMLAGADVHARDPRRGMSPQEWATYTGRVDAVR
LMQRLLERPCPEQFWEKYRPE
 

Matching transcripts

ANKRD33B-001 - ENSP00000296657 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:600 

Literature conformity

No avaliable gene/protein characterization data 

RNA consistency

Not done 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line HeLa shows positivity in mitochondria.
More information
 

Antibody dilution

1:118 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

 

Description

 

Target mass (kDa)

54.0 

Antibody dilution

1:390 

Validation WB

Current setup is not applicable due to low RNA count 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:4700 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ANKRD33B-001