CACNB2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035325

 

Antibody HPA035326

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035325 HPA035326 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

13 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

50 80 

Antigen sequence

SLGDIVPSSRKSTPPSSAIDIDATGLDAEENDIPANHRSPKPSANSVTSP
 
RDSAYVEPKEDYSHDHVDHYASHRDHNHRDETHGSSDHRHRESRHRSRDV
DREQDHNECNKQRSRHKSKDRYCEKDGEVI
 

Matching transcripts

CACNB2-001 - ENSP00000320025 [100%]
CACNB2-002 - ENSP00000282343 [100%]
CACNB2-003 - ENSP00000379821 [100%]
CACNB2-004 - ENSP00000366532 [100%]
CACNB2-007 - ENSP00000366546 [100%]
 CACNB2-001 - ENSP00000320025 [100%]
CACNB2-002 - ENSP00000282343 [100%]
CACNB2-003 - ENSP00000379821 [100%]
CACNB2-004 - ENSP00000366532 [100%]
CACNB2-005 - ENSP00000366536 [100%]
CACNB2-007 - ENSP00000366546 [100%]
CACNB2-008 - ENSP00000344474 [100%]
CACNB2-009 - ENSP00000366545 [100%]
CACNB2-010 - ENSP00000366548 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
More information
 Immunohistochemical staining of human lateral ventricle shows strong positivity in neuronal cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:250 1:200 

Literature conformity

Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not at all consistent with RNAseq data Mainly not consistent with RNAseq data 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

73.6, 70.8, 68.8, 68.2, 68.1 73.6, 71.1, 70.8, 68.8, 68.3, 68.2, 68.1, 64.0, 45.8 

Antibody dilution

1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

 1:81 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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CACNB2-001
 
CACNB2-002
 
CACNB2-003
 
CACNB2-004
 
CACNB2-005
 
CACNB2-007
 
CACNB2-008
 
CACNB2-009
 
CACNB2-010