CARNS1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA038569

 

Antibody HPA038570

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA038569 HPA038570 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

98 91 

Antigen sequence

LMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAP
EQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFY
 
VGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPY
CSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK
 

Matching transcripts

CARNS1-001 - ENSP00000308268 [100%]
CARNS1-002 - ENSP00000431670 [100%]
CARNS1-201 - ENSP00000401519 [100%]
CARNS1-202 - ENSP00000389009 [100%]
 CARNS1-001 - ENSP00000308268 [100%]
CARNS1-002 - ENSP00000431670 [100%]
CARNS1-201 - ENSP00000401519 [100%]
CARNS1-202 - ENSP00000389009 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.
More information
 Immunohistochemical staining of human cerebral cortex shows distinct positivity in glial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:250 1:200 

Validation IH

Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns and there is no available gene/protein characterization data 

         Western Blot

Image

  

Description

  

Target mass (kDa)

101.3, 98.7, 88.5 101.3, 98.7, 88.5 

Antibody dilution

1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CARNS1-001
 
CARNS1-002
 
CARNS1-201
 
CARNS1-202