PARP10

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA028122

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA028122 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

91 

Antigen sequence

PHYDILEPEELAENTSGGDHPSTQGPRATKHALLRTGGLVTALQGAGTVT
MGSGEEPGQSGTSLRTGPMVQGRGIMTTGSGQEPGQSGTSL
 

Matching transcripts

PARP10-001 - ENSP00000325618 [99%]
PARP10-004 - ENSP00000431620 [99%]
PARP10-005 - ENSP00000314320 [99%]
PARP10-006 - ENSP00000432733 [99%]
PARP10-008 - ENSP00000434776 [99%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:35 

Literature conformity

Partly consistent with extensive gene/protein characterization data 

RNA consistency

Not done 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in the Golgi apparatus & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:7 

Validation IF

Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

111.4, 110.0, 109.1, 81.5, 35.4 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PARP10-001
 
PARP10-004
 
PARP10-005
 
PARP10-006
 
PARP10-008