PTPN11

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB005377

 

ANTIBODY INFORMATION

Provider

Santa Cruz Biotechnology
 

Product name

sc-280 

Host species

Rabbit 

Clonality

msAb 

Purity

Affinity 

Released in version

3 

ANTIGEN INFORMATION

Antigen

Synthetic peptide 

Length (aa)

 

Antigen sequence

 

Matching transcripts

 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:350 

Literature conformity

Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNAseq data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, nucleoli, cytoplasm & actin filaments.
More information
 

Antibody dilution

1:87 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

68.0, 52.8, 12.2 

Antibody dilution

1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 
 

ANTIGEN VIEW

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 Not available