BRCC3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA048737

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA048737 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

137 

Antigen sequence

PSHVDVRTQAMYQMMDQGFVGLIFSCFIEDKNTKTGRVLYTCFQSIQAQK
SSEYERIEIPIHIVPHVTIGKVCLESAVELPKILCQEEQDAYRRIHSLTH
LDSVTKIHNGSVFTKNLCSQMSAVSGPLLQWLEDRLE
 

Matching transcripts

BRCC3-002 - ENSP00000328641 [100%]
BRCC3-006 - ENSP00000344103 [100%]
BRCC3-001 - ENSP00000358474 [85%]
BRCC3-201 - ENSP00000381998 [84%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
More information
 

Antibody dilution

1:102 

Validation IF

Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

36.1, 33.2, 33.1 

Antibody dilution

1:510 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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BRCC3-001
 
BRCC3-002
 
BRCC3-006
 
BRCC3-201