H2AFX

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA041189

 

Antibody CAB012264

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Abcam plc
 

Product name

HPA041189 ab10475 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

9 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

26  

Antigen sequence

AVRNDEELNKLLGGVTIAQGGVLPNI
 
 

Matching transcripts

H2AFX-001 - ENSP00000434024 [96%]
H2AFX-002 - ENSP00000364310 [96%]
  

Other gene match

H2AFY2 - ENSG00000099284 [81%]
H2AFY - ENSG00000113648 [85%]
HIST1H2AB - ENSG00000137259 [92%]
HIST1H2AA - ENSG00000164508 [96%]
HIST1H2AE - ENSG00000168274 [92%]
HIST1H2AC - ENSG00000180573 [92%]
HIST3H2A - ENSG00000181218 [92%]
HIST1H2AJ - ENSG00000182611 [92%]
HIST2H2AA3 - ENSG00000183558 [92%]
HIST2H2AC - ENSG00000184260 [92%]
HIST2H2AB - ENSG00000184270 [100%]
HIST1H2AK - ENSG00000184348 [92%]
HIST1H2AH - ENSG00000184825 [92%]
HIST1H2AI - ENSG00000196747 [92%]
HIST1H2AG - ENSG00000196787 [92%]
HIST1H2AD - ENSG00000196866 [92%]
HIST1H2AL - ENSG00000198374 [92%]
HIST2H2AA4 - ENSG00000203812 [92%]
HIST1H2AM - ENSG00000233224 [92%]
H2AFJ - ENSG00000246705 [92%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
More information
 Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:500 1:500 

Validation IH

Supportive: The multi-targeting antibody yielding a staining pattern consistent with gene/protein characterization data for all of the genes Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

40.1, 39.6, 39.5, 39.2, 17.6, 15.1, 14.2, 14.1, 14.0, 13.9, 10.0 15.1 

Antibody dilution

1:250 1:500 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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H2AFX-001
 
H2AFX-002