PAX5

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody CAB026269

 

Antibody CAB026869

 

ANTIBODY INFORMATION

Provider

Lab Vision/NeoMarkers
 R&D systems
 

Product name

RM-9133-S0 AF3487 

Host species

Rabbit Goat 

Clonality

mAb pAb 

Purity

Protein A/G Protein A/G 

Released in version

5 5 

ANTIGEN INFORMATION

Antigen

Synthetic peptide Recombinant protein 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human tonsil shows distinct nuclear positivity in the white pulp.
More information
 Immunohistochemical staining of human tonsil shows distinct nuclear positivity in reaction center cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:80 1:120 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

42.1, 38.9, 38.8, 37.8, 35.5, 35.3, 34.6, 34.1, 32.5, 31.8, 31.4, 30.3, 23.8, 23.7, 6.0 42.1, 38.9, 38.8, 37.8, 35.5, 35.3, 34.6, 34.1, 32.5, 31.8, 31.4, 30.3, 23.8, 23.7, 6.0 

Antibody dilution

1:500 1:500 

Validation WB

Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 
 

ANTIGEN VIEW

? »
 Not available