ERI2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035384

 

Antibody HPA035385

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035384 HPA035385 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

106 100 

Antigen sequence

TVPISDSDLEISFNSGERLMVLKELEMSSHENFGDIEETPQKSETSKSIV
YKSPHTTIYNVKEAKDPGSDISAFKLPEHKSSTFNRVNANMSHPLVLGKH
PLLSGG
 
ALLAWKMIRDGCVMKITRSLNKVPTKKNFSILARNLNTIQVEEMSACNIS
IQGPSIYNKEPKNIINPHEKVQMKSICANSPIKAQQDQLQVKNNIKASLH
 

Matching transcripts

ERI2-003 - ENSP00000454661 [100%]
ERI2-014 - ENSP00000455982 [100%]
ERI2-201 - ENSP00000350651 [100%]
ERI2-202 - ENSP00000373996 [100%]
 ERI2-003 - ENSP00000454661 [100%]
ERI2-014 - ENSP00000455982 [100%]
ERI2-201 - ENSP00000350651 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
More information
 Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in distal tubules.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:500 1:60 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & cytokinetic bridge.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in the Golgi apparatus & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:200 1:6 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

77.4, 66.8, 47.2 77.4, 66.8 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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ERI2-003
 
ERI2-014
 
ERI2-201
 
ERI2-202