NPIPB9

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA011188

 

Antibody HPA053611

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA011188 HPA053611 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

43 26 

Antigen sequence

EQSPKPKRQREAEAQQLPKPKRRRLSKLRTRHCTQAWAIRINP
 
NRMGHQPPPPTQQHSIIDNSLSLKTP
 

Matching transcripts

NPIPB9-001 - ENSP00000446902 [100%]
NPIPB9-002 - ENSP00000350444 [100%]
 NPIPB9-001 - ENSP00000446902 [92%]
NPIPB9-002 - ENSP00000350444 [92%]
 

Other gene match

NPIPB15 - ENSG00000196436 [100%]
NPIPB6 - ENSG00000198156 [100%]
NPIPB7 - ENSG00000233232 [100%]
NPIPB8 - ENSG00000255524 [100%]
 NPIP - ENSG00000169203 [96%]
NPIPB3 - ENSG00000169246 [96%]
NPIPA1 - ENSG00000183426 [100%]
NPIPA5 - ENSG00000183793 [100%]
NPIPA1 - ENSG00000183889 [100%]
NPIPB4 - ENSG00000185864 [96%]
NPIPB15 - ENSG00000196436 [92%]
NPIP - ENSG00000198064 [96%]
NPIPB6 - ENSG00000198156 [92%]
NPIPA8 - ENSG00000214940 [100%]
NPIPA7 - ENSG00000214967 [100%]
NPIPA3 - ENSG00000224712 [100%]
NPIPA1 - ENSG00000233024 [100%]
NPIPB7 - ENSG00000233232 [92%]
RP11-166B2.1 - ENSG00000234719 [96%]
NPIPB5 - ENSG00000243716 [96%]
NPIPB11 - ENSG00000254206 [92%]
NPIPA2 - ENSG00000254852 [100%]
NPIPB8 - ENSG00000255524 [92%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
More information
 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:100 1:20 

Literature conformity

No avaliable gene/protein characterization data No avaliable gene/protein characterization data 

RNA consistency

Not consistent with RNA expression data Not consistent with RNA expression data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

51.3, 49.6, 49.2, 48.1, 47.7, 47.2, 36.2 129.2, 126.7, 126.5, 126.0, 125.2, 67.1, 65.9, 51.3, 49.6, 49.2, 48.1, 47.7, 47.2, 45.8, 45.6, 42.3, 42.2, 42.1, 40.6, 40.1, 40.0, 36.2, 35.3, 16.7 

Antibody dilution

1:250 1:220 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:1750 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
NPIPB9-001
 
NPIPB9-002