MDM4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA018919

 

Antibody HPA048821

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA018919 HPA048821 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

71 94 

Antigen sequence

SFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQ
SAEESSTSRKRTTEDDIPTLP
 
LSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTH
SLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENS
 

Matching transcripts

MDM4-001 - ENSP00000356150 [100%]
MDM4-203 - ENSP00000396840 [100%]
 MDM4-001 - ENSP00000356150 [100%]
MDM4-007 - ENSP00000395254 [100%]
MDM4-203 - ENSP00000396840 [100%]
MDM4-205 - ENSP00000478080 [100%]
MDM4-206 - ENSP00000482388 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:65  

Literature conformity

Partly consistent with extensive gene/protein characterization data  

RNA consistency

Mainly consistent with RNA expression data  

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

 1:200 

Validation IF

 Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

54.9, 49.5 54.9, 49.5, 44.0, 29.9, 19.6 

Antibody dilution

1:250 1:1420 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MDM4-001
 
MDM4-007
 
MDM4-203
 
MDM4-205
 
MDM4-206