AGER

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA064436

 

Antibody CAB011682

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Abcam plc
 

Product name

HPA064436 ab30381 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

14 3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

22  

Antigen sequence

GVPNKVVEESRRSRKRPCEQEV
 
 

Matching transcripts

AGER-007 - ENSP00000364196 [100%]
AGER-202 - ENSP00000364210 [100%]
AGER-204 - ENSP00000387887 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Application not done for this antibody. Immunohistochemical staining of human lung shows strong membranous positivity in endothelial cells and pneumocytes.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:500 

Literature conformity

 Partly consistent with extensive gene/protein characterization data 

RNA consistency

 Mainly consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line MCF7 shows positivity in nucleoli & cell junctions.
More information
 Application not done for this antibody. 

Antibody dilution

1:94  

Validation IF

Non-supportive: The subcellular location is not consistent with experimental gene/protein characterization data, or conflicting with an independent antibody.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

44.8, 39.0, 25.2 44.8, 42.8, 39.0, 37.0, 36.2, 31.3, 25.2, 16.3, 14.0, 13.8 

Antibody dilution

1:310 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3750  

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).  
 

ANTIGEN VIEW

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AGER-007
 
AGER-202
 
AGER-204