KRTAP9-6

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042482

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA042482 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

29 

Antigen sequence

SSCAPVYCRRTCYHPTSVCLPGCLNQSCG
 

Matching transcripts

KRTAP9-6-001 - ENSP00000375150 [97%]
 

Other gene match

KRTAP9-7 - ENSG00000180386 [93%]
KRTAP9-8 - ENSG00000187272 [97%]
KRTAP9-9 - ENSG00000198083 [93%]
KRTAP9-3 - ENSG00000204873 [100%]
KRTAP9-2 - ENSG00000239886 [93%]
KRTAP9-4 - ENSG00000241595 [93%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in a subset of glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:35 

Validation IH

Uncertain: The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes 

         Western Blot

Image

 

Description

 

Target mass (kDa)

18.3, 17.8, 17.7, 16.9, 16.8, 16.7, 16.4 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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KRTAP9-6-001