CLIC1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB020825

 

Antibody CAB040557

 

ANTIBODY INFORMATION

Provider

SDIX
 NCI-CPTC
 

Product name

3262.00.02 CPTC-CLIC1-1 

Host species

Rabbit Mouse 

Clonality

msAb mAb 

Purity

Affinity Protein A/G 

Released in version

5 7 

ANTIGEN INFORMATION

Antigen

Genetic immunization Recombinant protein 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
More information
 Immunohistochemical staining of human kidney shows strong positivity of luminal membranes in renal tubules.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:350 1:650 

Validation IH

Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns which are partly consistent with or contradicted by limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding similar or partly similar staining patterns which are partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

26.9 26.9 

Antibody dilution

1:500 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Immunofluorescence

Image

  

Description

 Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
More information
 

Antibody dilution

 1:200 

Validation IF

 Supportive: The subcellular location is supported by literature. 
 

ANTIGEN VIEW

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 Not available