CT47A11

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042483

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA042483 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

28 

Antigen sequence

PEEVTKSQPEKWDEEAQDAAGEEEKEQE
 

Matching transcripts

CT47A11-001 - ENSP00000410400 [100%]
 

Other gene match

CT47A10 - ENSG00000224089 [100%]
CT47A6 - ENSG00000226023 [100%]
CT47A9 - ENSG00000226600 [100%]
CT47A12 - ENSG00000226685 [100%]
CT47A7 - ENSG00000228517 [100%]
CT47A8 - ENSG00000230347 [100%]
CT47A4 - ENSG00000230594 [100%]
CT47A3 - ENSG00000236126 [100%]
CT47A1 - ENSG00000236371 [100%]
CT47B1 - ENSG00000236446 [100%]
CT47A5 - ENSG00000237957 [100%]
CT47A2 - ENSG00000242362 [100%]
RP1-321E8.5 - ENSG00000278646 [89%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human testis shows strong cytoplasmic and nucleolar positivity in cells in seminiferus ducts.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:750 

Literature conformity

Consistent with gene/protein characterization data 

RNA consistency

Mainly not consistent with RNA expression data 

         Western Blot

Image

 

Description

 

Target mass (kDa)

31.3, 30.1, 4.5 

Antibody dilution

1:250 

Validation WB

Current setup is not applicable due to low RNA count 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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CT47A11-001