The first version of the HPA (www.proteinatlas.org) was based on a map of expression and localization profiles in 44 normal human tissues and 20 different cancers. The first version of this publicly available database contained approximately 400,000 high-resolution images using 700 antibodies generated in-house. Each image was annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues.
Key publication
- Uhlén M et al., A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol Cell Proteomics. (2005)
PubMed: 16127175 DOI: 10.1074/mcp.M500279-MCP200
Other selected publications
-
Agaton C et al., Selective enrichment of monospecific polyclonal antibodies for antibody-based proteomics efforts. J Chromatogr A (2004)
PubMed: 15317410
-
Nilsson P et al., Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling. Proteomics. (2005)
PubMed: 16237735 DOI: 10.1002/pmic.200500072
-
Uhlen M et al., Antibody-based proteomics for human tissue profiling. Mol Cell Proteomics. (2005)
PubMed: 15695805 DOI: 10.1074/mcp.R500009-MCP200
-
Andersson AC et al., Analysis of protein expression in cell microarrays: a tool for antibody-based proteomics. J Histochem Cytochem. (2006)
PubMed: 16957166 DOI: 10.1369/jhc.6A7001.2006
-
Berglund L et al., A genecentric Human Protein Atlas for expression profiles based on antibodies. Mol Cell Proteomics. (2008)
PubMed: 18669619 DOI: 10.1074/mcp.R800013-MCP200
Figure legend: The launch of the HPA portal. (A) The home page and (B) the surgical specimens analyzed in this first version. In total, 144 different tissue cores representing 44 different tissue types were assembled and stained using antibody-based immunohistochemistry.
Key facts
- The first version of the HPA was launched in 2005 at the Human Proteome Organization (HUPO) conference in Munich, Germany
- The first version included analysis using 718 antibodies against 650 human proteins
- The knowledge-based portal contained 413,568 images, all annotated by certified pathologists
- A search for “Human Protein Atlas” in Google Scholar now yields more than 10,000 publications
The Human Protein Atlas project is funded
