The portal Antibodypedia (www.antibodypedia.com) was launched in 2008 to allow sharing of information regarding validation of antibodies. The database provides a resource of publicly available antibodies to human proteins with accompanying experimental evidence supporting an individual validation score for each antibody in an application-specific manner. The resource now contains information for antibodies corresponding to over 19,000 human protein targets.
Other selected publications
Cottingham K., Antibodypedia seeks to answer the question: "how good is that antibody?". J Proteome Res. (2008)
PubMed: 18767878 DOI: 10.1021/pr800626d
Jonasson K et al., The 6th HUPO Antibody Initiative (HAI) workshop: sharing data about affinity reagents and other recent developments. September 2009, Toronto, Canada. Proteomics. (2010)
PubMed: 20514648 DOI: 10.1002/pmic.201090040
Alm T et al., A chromosome-centric analysis of antibodies directed toward the human proteome using Antibodypedia. J Proteome Res. (2014)
PubMed: 24533432 DOI: 10.1021/pr4011525
Alm T et al., Introducing the Affinity Binder Knockdown Initiativeâ¿¿A publicâ¿¿private partnership for validation of affinity reagents. EuPA Open Proteom. (2016)
PubMed: 29900101 DOI: 10.1016/j.euprot.2016.01.002
Figure legend: Antibodypedia constitutes a tool for evidence-based selection of antibodies for research. The portal enables users to (A) search for antibodies targeting specific proteins for selected applications and (B) compare side-by-side experimental evidence for various antibodies to the same target [example showing three anti-epidermal growth factor receptor (EGFR) antibodies].
- Antibodypedia contains information about more than 4 million antibodies
- The database contains more than 2 million validation experiments
- More than half (55%) of the antibodies produced are validated for Western blot
- Other common applications are immunohistochemistry, immunocytochemistry, and flow cytometry